AtlantECO standards and best practice workshop- edition 2020
Time & Location
About the Event
AtlantECO will hold a standards & best practice workshop every year over the next four years to recommend sampling, biobanking and analysis protocols for the study of microbiomes, plastics and the plastisphere in marine, estuarine, sediment and atmospheric environments. Although the recommendations will be relevant to a wide range of applications (e.g. genomic observatories), the prime objective is to provide a coherent set of selected protocols that are adapted to the activities of AtlantECO, including dedicated cruises on oceanographic vessels and research sail boats, opportunistic cruises, the all-Atlantic Ocean Sampling Day, and the citizen sail-4-science.
Sessions will consist of a mix of flash presentations and discussions. We encourage participants to prepare flash presentations (max 5 slides per topic and as many topics as you wish) that will be used in an ad hoc fashion to feed the discussions. Session chairs may also contact you regarding a more targeted contribution.
All slides will be made available online by Wednesday 4th November and all participants are expected to go through the slides before the session (details in the registration form).
Following the workshop, a handbook will be drafted, and all participants will be invited to review it online. A first version of the Handbook will be published in open access in Zenodo in February 2021 and will be updated every year in order to incorporate changes and additions discussed in the next editions of the workshop.
The format of the workshop will be five VIRTUAL sessions from Monday to Friday:
Monday 9 November, 15-17h CET (Paris time)
INTRODUCTION - Plenary session
Chair: UNESCO-IOC (Ocean Best Practices System)
· Summary of AtlantECO and augmented observations planned in 2021-24 (Stéphane Pesant)
· Outlook on standards and best practices in marine science (Pier Luigi Buttigieg)
· Report on the January 2020 OCB Workshop on 'omics intercalibration & strandardisation (Scott Gifford, TBC)
· Overview of the workshop sessions (session chairs)
Wednesday 11 November, 14-18h CET (Paris time)
SESSION 2 - MICROBIOMES SAMPLING & IMAGING ANALYSIS PROTOCOLS
Chair: Stéphane Pesant (EMBL-EBI) & Fabien Lombard (Sorbonne Université)
Short description: A mix of flash presentations and discussions about sampling devices, filtration volume and time, size-fractions, pre-filtration, replication, etc. While the focus is on ocean microbiomes, we will address sampling methods in other environments such as rivers, sediments and aerosols. Imaging methods covering the full size-spectrum of microbiomes will be presented and discussed.
Thursday 12 November, 14-18h CET (Paris time)
SESSION 3 - PLASTICS SAMPLING, ANALYSIS & EXPERIMENTAL PROTOCOLS
Chairs: Linda Amaral Zettler (NIOZ), Alice Horton (NOC) & Jeff Ghiglione (CNRS)
Short description: A mix of flash presentations and discussions covering techniques and best practice for microplastic sampling and analysis within aquatic environments, including sample preservation methods to retain biofilms and plastisphere communities, and experimental protocols for assessing microplastic-organism interactions.
Friday 13 November, 15-17h CET (Paris time)
SESSIONS REPORTING & NEXT STEPS
Chair: Nicolas Pade (EMBRC)
Short description: Recommendations from the three sessions will be presented and we will discuss the timeline and review process of the Handbook. Contributions to a special issue in Frontiers in Microbiology will also be discussed.